2026-07-28T18:14:43Zhttps://www.radar-service.eu/oai/OAIHandler

10.22000/3192023-11-15T14:40:18Z

10.22000/319 Feiner, Alexander Alexander Feiner 0000-0001-6564-8826 Hopsteiner, Simon H. Steiner, Hopfen, GmbH, 84048 Mainburg, Germany & Leibniz Institute of Plant Biochemistry, 06120 Halle/Saale, Germany Pitra, Nicholi Nicholi Pitra 0000-0001-8642-5138 Hopsteiner, S.S. Steiner, Inc., New York, New York 10065, USA Matthews, Paul Paul Matthews Hopsteiner, S.S. Steiner, Inc., New York, New York 10065, USA Pillen, Klaus Klaus Pillen Martin-Luther University, 06120 Halle/Saale, Germany Wessjohann, Ludger A. Ludger A. Wessjohann 0000-0003-2060-8235 Leibniz Institute of Plant Biochemistry, 06120 Halle/Saale, Germany Riewe, David David Riewe 0000-0002-9095-5518 Leibniz Institute of Plant Genetics and Crop Plant Research, 06466 Gatersleben, Germany & Julius Kuehn-Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Ecological Chemistry, Plant Analysis and Stored Product Protection, 14195 Berlin, Germany Simon H. Steiner, Hopfen, GmbH, Mainburg, Germany,Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany,Julius Kuehn-Institute (JKI), Berlin, Germany,Leibniz Institute of Plant Biochemistry (IPB), Halle (Saale), Germany 2015 2020 Biochemistry Agriculture downy mildew resistance untargeted metabolomics genome-wide association study phenylpropanoids hop oomycete MS Peak table Open Access Creative Commons Attribution 4.0 International Simon H. Steiner, Hopfen, GmbH Downy mildew in hop (Humulus lupulus L.) is caused by Pseudoperonospora humuli and generates significant losses in quality and yield. To identify the biochemical processes that confer natural downy mildew resistance (DMR), a metabolome- and genome-wide association study was performed. Inoculation of a high density genotyped F1 hop population (n=192) with the obligate biotrophic oomycete P. humuli led to variation in both the levels of thousands of specialized metabolites and DMR. We observed that metabolites of almost all major phytochemical classes were induced 48 hours after inoculation. But only a small number of metabolites were found to be correlated with DMR and these were enriched with phenylpropanoids. These metabolites were also correlated with DMR when measured from the non-infected control set. A genome-wide association study revealed co-localization of the major DMR loci and the phenylpropanoid pathway markers indicating that the major contribution to resistance is mediated by these metabolites in a heritable manner. The application of three putative prophylactic phenylpropanoids led to a reduced degree of leaf infection in susceptible genotypes, confirming their protective activity either directly or as precursors of active compounds. Humulus lupulus LC/MS-qToF application/x-tar