2026-06-27T08:25:25Zhttps://www.radar-service.eu/oai/OAIHandler

10.22000/4002023-11-15T14:40:19Z

10.22000/400 Schnabel, Arianne Arianne Schnabel 0000-0002-2172-2905 Leibniz Institute of Plant Biochemistry, Dept. Cell and Metabolic Biology, 06120 Halle (Saale), Germany Athmer, Benedikt Benedikt Athmer 0000-0002-0234-4398 Leibniz Institute of Plant Biochemistry, Dept. Cell and Metabolic Biology, 06120 Halle (Saale), Germany Manke, Kerstin Kerstin Manke Leibniz Institute of Plant Biochemistry, Dept. Cell and Metabolic Biology, 06120 Halle (Saale), Germany Schumacher, Frank Frank Schumacher Core Facility Vienna Botanical Gardens, Rennweg 14/2, 1030 Vienna, Austria Cotinguiba, Fernando Fernando Cotinguiba 0000-0002-7006-0768 Instituto de Pesquisas de Produtos Naturais (IPPN), Universidade Federal do Rio de Janeiro (UFRJ), Avenida Carlos Chagas Filho, 373, 21941-902, Rio de Janeiro/RJ, Brasil Vogt, Thomas Thomas Vogt 0000-0001-6155-3360 Leibniz Institute of Plant Biochemistry, Dept. Cell and Metabolic Biology, 06120 Halle (Saale), Germany Vogt, Thomas 2021 2021 Biochemistry Piper nigrum, raw data chromatograms, Excel data, sequence signals chromatograms, Excel files, raw data Open Access Creative Commons Attribution Share Alike 4.0 International Leibniz Institute of Plant Biochemistry, Dept. Cell and Metabolic Biology, 06120 Halle (Saale), Germany Black pepper (Piper nigrum L.) is the world’s most popular spice and is also used as an ingredient in traditional medicine. Its pungent perception is due to the interaction of its major compound, 1-piperoyl-piperidine (piperine) with the human TRPV-1 or vanilloid receptor. We now identified the hitherto concealed enzymatic formation of piperine from piperoyl coenzyme A and piperidine based on a differential RNA-Seq approach from developing black pepper fruits. This enzyme is described as piperine synthase (piperoyl-CoA:piperidine piperoyl transferase) and is a member of the BAHD-type of acyltransferases encoded by a gene that is preferentially expressed in immature fruits. A second BAHD-type enzyme, also highly expressed in immature black pepper fruits has a rather promiscuous substrate specificity combining diverse CoA-esters with aliphatic and aromatic amines with similar efficiencies, and was termed piperamide synthase. Recombinant piperine and piperamide synthases are members of a small BAHD-gene family in black pepper. They can be used to facilitate the microbial production of a broad range of medicinally relevant aliphatic and aromatic amides piperamides based on a wide array CoA-donors and amine-derived acceptors offering widespread applications. Waters e2695 chromatography work station equipped with a photodiode array detector (PDA) and a QDA-mass detector (Waters) DOI Journal Article Deutsche Forschungsgemeinschaft (DFG) grid.424150.6 VO719/15-1 Deutsche Forschungsgemeinschaft (DFG) grid.424150.6 VO719/15-2 application/x-tar