2026-10-12T01:48:16Zhttps://www.radar-service.eu/oai/OAIHandler

10.35097/aeiBlRjlEYdPYTaq2026-10-09T10:48:26Zradar4kit

https://dx.doi.org/10.35097/aeiBlRjlEYdPYTaq Narwal, Pooja Lorz, Nils Minaei, Masoud Jannin, Sami Kouril, Karel Gossert, Alvar Diego Meier, Benno Bullet-DNP enables NMR Spectroscopy of Pyruvate and Amino Acids at Nanomolar to Low Micromolar Concentrations (Datasets) Karlsruhe Institute of Technology 2024 Biology dataset Dataset info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/legalcode Hyperpolarized pyruvate is a widely used marker to track metabolism in vivo and a benchmark molecule for hyperpolarization methods. Here, we show how a combination of improved bullet-DNP instrumentation, an optimized sample preparation and a further sensitivity increase via a 13C–1H polarization transfer after dissolution enable the observation of pyruvate at a concentration of 250 nM immediately after dissolution. At this concentration, the experiment employs a total mass of pyruvate of only 20 ng or 180 pmol. If the concentration is increased to 45 μM, pyruvate may be detected 1 min after dissolution with a signal-to-noise ratio exceeding 50. The procedure can be extended to observe a mixture of amino acids at low micromolar concentrations. Contains TopSpin raw data and high-resolution figures https://publikationen.bibliothek.kit.edu/1000172444 application/x-tar