2026-10-13T07:14:19Zhttps://www.radar-service.eu/oai/OAIHandler10.35097/pn5npep7vfjn92fe2026-10-09T10:59:31Zradar4kit
https://dx.doi.org/10.35097/pn5npep7vfjn92fe
Lohfink, Kim Carina
Kirsch, Michel
Nirschl, Hermann
Online measurement data of continuous aqueous two-phase flotation (ATPF) for enzyme purification
Karlsruhe Institute of Technology
2024
Engineering
dataset
Dataset
info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/4.0/legalcode
- The .csv files contain experimental data obtained from ATPF experiments at laboratory scale.
- The flotation tank used contains 700 ml of the aqueous two-phase system (ATPS). The phase forming components were polyethylene glycol 1000 and trisodium citrate dihydrate.
- Phospholipase A2 was used as the model enzyme and the model suspension was mixed with the bottom phase.
- The flotation tank was conceptually divided into three compartments, each compartment containing a gassing unit and an electrical conductivity probe. The electrical conductivity probes were inserted 1 cm below the phase interface.
- An UV/Vis spectrometer with flow cell was implemented at the top phase outlet to obtain the top phase concentration in real-time. The bottom phase concentration was calculated via a mass balance equation.
- The file names contain the process parameters in the following order: feed concentration_gas flow rate combination (Vd_1-Vd_2-Vd_3, see also below)_residence time_volume ratio (top to bottom phase)_experiment number.
- The .csv files contain the following experimental data:
- t: time in min
- R_top, R_bot: top and bottom phase recirculation (R = 1 for full recirculation, R = 0 for no recirculation)
- c_bot_3, c_top_3: enzyme concentration obtained from UV/Vis measurements in % (w/w).
- kappa_bot_1, kappa_bot_2, kappa_bot_3: Electrical conductivity measurements in all three compartments in mS/cm.
- Qd_bot, Qd_top: Volume flows of top and bottom phase in ml/min.
- Vd_1, Vd_2, Vd_3: Gas volume flows of the three gassing units in ml/min.
https://publikationen.bibliothek.kit.edu/1000174648
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