Alternativer Identifier:
-
Verwandter Identifier:
-
Ersteller/Autor:
Thiele, Christoph [Rheinische Friedrich-Wilhelms-Universität Bonn, LIMES Institut] https://orcid.org/0000-0001-5161-2558
Beitragende:
(Project Leader) Thiele, Christoph https://orcid.org/0000-0001-5161-2558
(Researcher) Wunderling, Klaus https://orcid.org/0000-0003-1237-7510
(Researcher) Leyendecker, Philipp https://orcid.org/0000-0002-4709-9218
Weitere Titel:
-
Beschreibung:
(Table Of Contents) Abstact of the publication: Cellular lipid metabolism is a complex network process comprising dozens of enzymes, multiple organelles and more than a thousand lipid species. Tracing metabolic reactions in this network is a major technological and scientific challenge. Using a novel click-chemistry mass spectrometry reporter strategy, we have developed a specific, highly sensitive and robust tracing procedure for alkyne-labeled lipids. The method enables sample multiplexing, saving time and costs and improving sample comparison. We demonstrate this by a time-resolved analysis of hepatocyte glycerolipid metabolism with parallel quantitiative monitoring of 120 labeled lipid species. The sub-femtomole sensitivity enabled the first single cell analysis of fatty acid incorporation into neutral and membrane lipids. The results demonstrate the robustness of lipid homeostasis at the single cell level. Content: This archive contains the primary Mass Spectrometry .raw files of all relevant data shown in the paper. Further, it contains the masterscan .sc files of the LipidXplorer software that result from the import of the .raw files into the software. Thes .sc files can be searched using LipidXplorer, using the .mfql files that are also part of this archive. Data are organized in folders according to the Figures and Supplementary Items of the original publication. Each folder contains a separate readme file that explaines the type of files, their origin and possible use.
Schlagworte:
-
Zugehörige Informationen:
-
Sprache:
-
Herausgeber:
Thiele, Christoph
Erstellungsjahr:
Fachgebiet:
Biochemistry
Objekttyp:
Dataset
Datenquelle:
-
Verwendete Software:
-
Datenverarbeitung:
-
Erscheinungsjahr:
Rechteinhaber:
Christoph Thiele
Förderung:
-
Name Speichervolumen Metadaten Upload

Zugriffe der letzten sechs Monate

Aufrufe der Datenpaket-Seite

135


Downloads des Datenpakets

0


Gesamtstatistik

Zeitraum Aufrufe der Datenpaket-Seite Datenpaket heruntergeladen
Jun 2020 5 0
Mai 2020 20 0
Apr 2020 19 0
Mär 2020 19 0
Feb 2020 33 0
Jan 2020 39 0
Vorher 225 8
Gesamt 360 8
Status:
PUBLISHED
Eingestellt von:
cthieleLIMES
Erstellt am:
Archivierungsdatum:
2019-10-14
Archivgröße:
3,7 GB
Archiversteller:
cthieleLIMES
Archiv-Prüfsumme:
d6a4d58892952c5b9a3202ce28109fcd (MD5)
Ende des Embargo-Zeitraums:
-
Publikationsdatum: 2019-10-14
Datenpaket herunterladen
Herunterladen (3,7 GB)
Metadaten herunterladen
Statistiken
360
Views
8
Downloads
Lizenz
Dieses Werk ist lizenziert unter
CC BY-NC-SA 4.0 Attribution-NonCommercial-ShareAlike
Datenpaket zitieren
Thiele, Christoph (2019): data archive for: Thiele, Wunderling, Leyendecker (2019) Multiplexed and single cell tracing of lipid metabolism, Nature Methods, https://doi.org/10.1038/s41592-019-0593-6. Thiele, Christoph.