Persistenter Identifikator:
(DOI) 10.22000/251
Alternativer Identifier:
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Verwandter Identifier:
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Ersteller/Autor:
Thiele, Christoph [Rheinische Friedrich-Wilhelms-Universität Bonn, LIMES Institut] https://orcid.org/0000-0001-5161-2558
Beitragende:
(Project Leader) Thiele, Christoph https://orcid.org/0000-0001-5161-2558
(Researcher) Wunderling, Klaus https://orcid.org/0000-0003-1237-7510
(Researcher) Leyendecker, Philipp https://orcid.org/0000-0002-4709-9218
Titel:
data archive for: Thiele, Wunderling, Leyendecker (2019) Multiplexed and single cell tracing of lipid metabolism, Nature Methods, https://doi.org/10.1038/s41592-019-0593-6
Weitere Titel:
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Beschreibung:
(Table Of Contents) Abstact of the publication: Cellular lipid metabolism is a complex network process comprising dozens of enzymes, multiple organelles and more than a thousand lipid species. Tracing metabolic reactions in this network is a major technological and scientific challenge. Using a novel click-chemistry mass spectrometry reporter strategy, we have developed a specific, highly sensitive and robust tracing procedure for alkyne-labeled lipids. The method enables sample multiplexing, saving time and costs and improving sample comparison. We demonstrate this by a time-resolved analysis of hepatocyte glycerolipid metabolism with parallel quantitiative monitoring of 120 labeled lipid species. The sub-femtomole sensitivity enabled the first single cell analysis of fatty acid incorporation into neutral and membrane lipids. The results demonstrate the robustness of lipid homeostasis at the single cell level. Content: This archive contains the primary Mass Spectrometry .raw files of all relevant data shown in the paper. Further, it contains the masterscan .sc files of the LipidXplorer software that result from the import of the .raw files into the software. Thes .sc files can be searched using LipidXplorer, using the .mfql files that are also part of this archive. Data are organized in folders according to the Figures and Supplementary Items of the original publication. Each folder contains a separate readme file that explaines the type of files, their origin and possible use.
Schlagworte:
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Zugehörige Informationen:
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Sprache:
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Herausgeber:
Thiele, Christoph
Erstellungsjahr:
Fachgebiet:
Biochemistry
Objekttyp:
Dataset
Datenquelle:
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Verwendete Software:
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Datenverarbeitung:
-
Erscheinungsjahr:
Lizenz:
CC BY-NC-SA 4.0 Attribution-NonCommercial-ShareAlike
Rechteinhaber:
Christoph Thiele
Förderung:
-
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Status:
PUBLISHED
Eingestellt von:
cthieleLIMES
Erstellt am:
Archivierungsdatum:
2019-10-14
Archivgröße:
3,7 GB
Archiversteller:
cthieleLIMES
Archiv-Prüfsumme:
d6a4d58892952c5b9a3202ce28109fcd (MD5)
Ende des Embargo-Zeitraums:
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Datenpaket Status:
PUBLISHED
Dieses Datenpaket zitieren:
Thiele, Christoph (2019): data archive for: Thiele, Wunderling, Leyendecker (2019) Multiplexed and single cell tracing of lipid metabolism, Nature Methods, https://doi.org/10.1038/s41592-019-0593-6. Thiele, Christoph.
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