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  2. Supporting Information: A Transient Expression Tool Box for Anthocyanin Biosynthesis in Nicotiana benthamiana
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    Datenpaket: Supporting Information: A Transient Expression Tool Box for Anthocyanin Biosynthesis in Nicotiana benthamiana

    Alternativer Identifier:
    -
    Verwandter Identifier:
    (Is Supplement To) 10.1111/pbi.14261 - DOI
    Ersteller/in:
    Grützner, Ramona [Leibniz Institute of Plant Biochemistry, Dept. of Cell and Metabolic Biology, Weinberg 3, 06120 Halle (Saale), Germany]

    König, Kristin [Leibniz Institute of Plant Biochemistry, Dept. of Cell and Metabolic Biology, Weinberg 3, 06120 Halle (Saale), Germany]

    Horn, Claudia [Leibniz Institute of Plant Biochemistry, Dept. of Cell and Metabolic Biology, Weinberg 3, 06120 Halle (Saale), Germany]

    Engler, Carola [Icon Genetics GmbH, Weinbergweg 22, 06120 Halle (Saale), Germany]

    Laub, Annegret https://orcid.org/0000-0001-6633-3439 [Leibniz Institute of Plant Biochemistry, Dept. of Bioorganic Chemistry, Weinberg 3, 06120 Halle (Saale), Germany]

    Vogt, Thomas https://orcid.org/0000-0001-6155-3360 [Leibniz Institute of Plant Biochemistry, Dept. of Cell and Metabolic Biology, Weinberg 3, 06120 Halle (Saale), Germany]

    Marillonnet, Sylvestre https://orcid.org/0000-0002-4263-797X [Leibniz Institute of Plant Biochemistry, Dept. of Cell and Metabolic Biology, Weinberg 3, 06120 Halle (Saale), Germany]
    Beitragende:
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    Titel:
    Supporting Information: A Transient Expression Tool Box for Anthocyanin Biosynthesis in Nicotiana benthamiana
    Weitere Titel:
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    Beschreibung:
    (Abstract) Transient expression in Nicotiana benthamiana offers a robust platform for the rapid production of complex secondary metabolites. It has proven highly effective in helping identify genes associated with pathways responsible for synthesizing various valuable natural compounds. While this approach has seen considerable success, it has yet to be applied to uncovering genes involved in anthocyanin biosynthetic pathways. This is because only a single anthocyanin, delphinidin 3-O-rutinoside, can be produced in N. benthamiana by activation of anthocyanin biosynthesis using transcription factors. The production of other anthocyanins would necessitate the suppression of certain endogenous flavonoid biosynthesis genes while transiently expressing others. In this work, we present a series of tools for reconstitution of anthocyanin biosynthetic pathways in N. benthamiana leaves. These tools include constructs for expression or silencing of anthocyanin biosynthetic genes and a mutant N. benthamiana line generated using CRISPR. By infiltration of defined sets of constructs, the basic anthocyanins pelargonidin 3-O-glucoside, cyanidin 3-O-glucoside and delphinidin 3-O-glucoside could be obtained in high amount in a few days. Additionally, co-infiltration of supplementary pathway genes enabled the synthesis of more complex anthocyanins. These tools should be useful to identify genes involved in the biosynthesis of complex anthocyanins. They also make it possible to produce novel anthocyanins not found in nature. As an example, we reconstituted the pathway for biosynthesis of Arabidopsis anthocyanin A5, a cyanidin derivative, and achieved the biosynthesis of the pelargonidin and delphinidin variants of A5, pelargonidin A5 and delphinidin A5.
    Schlagworte:
    Anthocyanins
    plant secondary metabolites
    biosynthetic pathway
    transient expression
    Nicotiana benthamiana
    plant biotechnology
    synthetic biology
    Zugehörige Informationen:
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    Sprache:
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    Herausgeber/in:
    Leibniz Institute of Plant Biochemistry
    Erstellungsjahr:
    Fachgebiet:
    Biochemistry
    Objekttyp:
    Dataset
    Datenquelle:
    (Instrument) Waters e2695 chromatography work station
    (Instrument) photodiode array detector (PDA)
    (Instrument) QDA-mass detector (Waters)
    Verwendete Software:
    Software für Datenerhebung
    Software:
    Waters Empower - 3
    Alternative Software:
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    Verwendete Software:
    Software für Datenbearbeitung
    Software:
    Genious Prime Molecular Biology and Bioinformatics - unspecified
    Alternative Software:
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    Verwendete Software:
    Software für Datenbearbeitung
    Software:
    Microsoft Office - 2013
    Alternative Software:
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    Datenverarbeitung:
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    Erscheinungsjahr:
    Rechteinhaber/in:
    Leibniz Institute of Plant Biochemistry, Dept. of Cell and Metabolic Biology, Weinberg 3, 06120 Halle (Saale), Germany
    Förderung:
    -
    Name Speichervolumen Metadaten Upload Aktion
    Status:
    Publiziert
    Eingestellt von:
    1670fa88949cc897f41b44d58d08c11a
    Erstellt am:
    Archivierungsdatum:
    2023-12-06
    Archivgröße:
    1,0 GB
    Archiversteller:
    1670fa88949cc897f41b44d58d08c11a
    Archiv-Prüfsumme:
    a2095d7cac7a3eba2a2fdb6afaef9d76 (MD5)
    Embargo-Zeitraum:
    -