Alternativer Identifier:
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Verwandter Identifier:
Ersteller/in:
Lohfink, Kim Carina https://orcid.org/0009-0009-1321-7720 [Institut für Mechanische Verfahrenstechnik und Mechanik (MVM), Karlsruher Institut für Technologie (KIT)]

Kirsch, Michel [Institut für Mechanische Verfahrenstechnik und Mechanik (MVM), Karlsruher Institut für Technologie (KIT)]

Nirschl, Hermann [Institut für Mechanische Verfahrenstechnik und Mechanik (MVM), Karlsruher Institut für Technologie (KIT)]
Beitragende:
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Titel:
Online measurement data of continuous aqueous two-phase flotation (ATPF) for enzyme purification
Weitere Titel:
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Beschreibung:
(Technical Remarks) - The .csv files contain experimental data obtained from ATPF experiments at laboratory scale. - The flotation tank used contains 700 ml of the aqueous two-phase system (ATPS). The phase forming components were polyethylene glycol 1000 and trisodium citrate dihydrate. - Phospholipase A2 was used as the model enzyme and the model suspension was mixed with the bottom phase. - The flotation tank was conceptually divided into three compartments, each compartment containing a gassing unit and an electrical conductivity probe. The electrical conductivity probes were inserted 1 cm below the phase interface. - An UV/Vis spectrometer with flow cell was implemented at the top phase outlet to obtain the top phase concentration in real-time. The bottom phase concentration was calculated via a mass balance equation. - The file names contain the process parameters in the following order: feed concentration_gas flow rate combination (Vd_1-Vd_2-Vd_3, see also below)_residence time_volume ratio (top to bottom phase)_experiment number. - The .csv files contain the following experimental data: - t: time in min - R_top, R_bot: top and bottom phase recirculation (R = 1 for full recirculation, R = 0 for no recirculation) - c_bot_3, c_top_3: enzyme concentration obtained from UV/Vis measurements in % (w/w). - kappa_bot_1, kappa_bot_2, kappa_bot_3: Electrical conductivity measurements in all three compartments in mS/cm. - Qd_bot, Qd_top: Volume flows of top and bottom phase in ml/min. - Vd_1, Vd_2, Vd_3: Gas volume flows of the three gassing units in ml/min.
Schlagworte:
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Zugehörige Informationen:
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Sprache:
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Erstellungsjahr:
Fachgebiet:
Engineering
Objekttyp:
Dataset
Datenquelle:
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Verwendete Software:
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Datenverarbeitung:
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Erscheinungsjahr:
Rechteinhaber/in:

Kirsch, Michel

Nirschl, Hermann
Förderung:
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Name Speichervolumen Metadaten Upload Aktion
Status:
Publiziert
Eingestellt von:
kitopen
Erstellt am:
Archivierungsdatum:
2024-10-08
Archivgröße:
170,5 kB
Archiversteller:
kitopen
Archiv-Prüfsumme:
3d520805a7c220ee3280d3e77e0546c3 (MD5)
Embargo-Zeitraum:
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