Alternativer Identifier:
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Verwandter Identifier:
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Ersteller/in:
Schnabel, Arianne https://orcid.org/0000-0002-2172-2905 [Leibniz Institute of Plant Biochemistry, Dept. Cell and Metabolic Biology, 06120 Halle (Saale), Germany]

Athmer, Benedikt https://orcid.org/0000-0002-0234-4398 [Leibniz Institute of Plant Biochemistry, Dept. Cell and Metabolic Biology, 06120 Halle (Saale), Germany]

Manke, Kerstin [Leibniz Institute of Plant Biochemistry, Dept. Cell and Metabolic Biology, 06120 Halle (Saale), Germany]

Schumacher, Frank [Core Facility Vienna Botanical Gardens, Rennweg 14/2, 1030 Vienna, Austria]

Cotinguiba, Fernando https://orcid.org/0000-0002-7006-0768 [Instituto de Pesquisas de Produtos Naturais (IPPN), Universidade Federal do Rio de Janeiro (UFRJ), Avenida Carlos Chagas Filho, 373, 21941-902, Rio de Janeiro/RJ, Brasil]

Vogt, Thomas https://orcid.org/0000-0001-6155-3360 [Leibniz Institute of Plant Biochemistry, Dept. Cell and Metabolic Biology, 06120 Halle (Saale), Germany]
Beitragende:
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Titel:
Nature Communications Biology RADAR - Feb 2021
Weitere Titel:
(Alternative Title) Identification and characterization of piperine synthase from black pepper (Piper nigrum L.)
Beschreibung:
(Abstract) Black pepper (Piper nigrum L.) is the world’s most popular spice and is also used as an ingredient in traditional medicine. Its pungent perception is due to the interaction of its major compound, 1-piperoyl-piperidine (piperine) with the human TRPV-1 or vanilloid receptor. We now identified the hitherto concealed enzymatic formation of piperine from piperoyl coenzyme A and piperidine based on a differential RNA-Seq approach from developing black pepper fruits. This enzyme is described as piperine synthase (piperoyl-CoA:piperidine piperoyl transferase) and is a member of the BAHD-type of acyltransferases encoded by a gene that is preferentially expressed in immature fruits. A second BAHD-type enzyme, also highly expressed in immature black pepper fruits has a rather promiscuous substrate specificity combining diverse CoA-esters with aliphatic and aromatic amines with similar efficiencies, and was termed piperamide synthase. Recombinant piperine and piperamide synthases are members of a small BAHD-gene family in black pepper. They can be used to facilitate the microbial production of a broad range of medicinally relevant aliphatic and aromatic amides piperamides based on a wide array CoA-donors and amine-derived acceptors offering widespread applications.
Schlagworte:
Piper nigrum, raw data chromatograms, Excel data, sequence signals
Zugehörige Informationen:
(10.1038/s42003-021-01967-9) DOI Journal Article
Sprache:
-
Herausgeber/in:
Vogt, Thomas
Erstellungsjahr:
Fachgebiet:
Biochemistry
Objekttyp:
(Dataset) chromatograms, Excel files, raw data
Datenquelle:
(Instrument) Waters e2695 chromatography work station equipped with a photodiode array detector (PDA) and a QDA-mass detector (Waters)
Verwendete Software:
Software für Datenbearbeitung
Software:
Microsoft Office - 2013
Systat SigmaPlot - 13.0
Serif Affinity Graphics - unknown
Biomatters Geneious - 11.0
DNAStar Lasergene Package incl. EditSeq. Protean, Megalign - 11
Alternative Software:
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Verwendete Software:
Software für Datenerhebung
Software:
Waters Embower - 3
BioRad CFX (qPCR) - 2.1
Alternative Software:
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Datenverarbeitung:
-
Erscheinungsjahr:
Rechteinhaber/in:
Leibniz Institute of Plant Biochemistry, Dept. Cell and Metabolic Biology, 06120 Halle (Saale), Germany
Förderung:
Deutsche Forschungsgemeinschaft (DFG) - (VO719/15-1)
Deutsche Forschungsgemeinschaft (DFG) - (VO719/15-2)
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Status:
Publiziert
Eingestellt von:
1670fa88949cc897f41b44d58d08c11a
Erstellt am:
Archivierungsdatum:
2021-04-01
Archivgröße:
613,3 MB
Archiversteller:
1670fa88949cc897f41b44d58d08c11a
Archiv-Prüfsumme:
87cbd3e63af4c8c3ff30700e5d6e6e26 (MD5)
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