Alternate identifier:
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Related identifier:
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Creator/Author:
Feiner, Alexander [Hopsteiner, Simon H. Steiner, Hopfen, GmbH, 84048 Mainburg, Germany & Leibniz Institute of Plant Biochemistry, 06120 Halle/Saale, Germany] https://orcid.org/0000-0001-6564-8826
Pitra, Nicholi [Hopsteiner, S.S. Steiner, Inc., New York, New York 10065, USA] https://orcid.org/0000-0001-8642-5138
Matthews, Paul [Hopsteiner, S.S. Steiner, Inc., New York, New York 10065, USA]
Pillen, Klaus [Martin-Luther University, 06120 Halle/Saale, Germany]
Wessjohann, Ludger A. [Leibniz Institute of Plant Biochemistry, 06120 Halle/Saale, Germany] https://orcid.org/0000-0003-2060-8235
Riewe, David [Leibniz Institute of Plant Genetics and Crop Plant Research, 06466 Gatersleben, Germany & Julius Kuehn-Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Ecological Chemistry, Plant Analysis and Stored Product Protection, 14195 Berlin, Germany] https://orcid.org/0000-0002-9095-5518
Contributors:
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Additional titles:
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Description:
(Abstract) Downy mildew in hop (Humulus lupulus L.) is caused by Pseudoperonospora humuli and generates significant losses in quality and yield. To identify the biochemical processes that confer natural downy mildew resistance (DMR), a metabolome- and genome-wide association study was performed. Inoculation of a high density genotyped F1 hop population (n=192) with the obligate biotrophic oomycete P. humuli led to variation in both the levels of thousands of specialized metabolites and DMR. We observed that metabolites of almost all major phytochemical classes were induced 48 hours after inoculation. But only a small number of metabolites were found to be correlated with DMR and these were enriched with phenylpropanoids. These metabolites were also correlated with DMR when measured from the non-infected control set. A genome-wide association study revealed co-localization of the major DMR loci and the phenylpropanoid pathway markers indicating that the major contribution to resistance is mediated by these metabolites in a heritable manner. The application of three putative prophylactic phenylpropanoids led to a reduced degree of leaf infection in susceptible genotypes, confirming their protective activity either directly or as precursors of active compounds.
Keywords:
downy mildew resistance
untargeted metabolomics
genome-wide association study
phenylpropanoids
hop
oomycete
Related information:
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Language:
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Publishers:
Simon H. Steiner, Hopfen, GmbH, Mainburg, Germany
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany
Julius Kuehn-Institute (JKI), Berlin, Germany
Leibniz Institute of Plant Biochemistry (IPB), Halle (Saale), Germany
Production year:
Subject areas:
Biochemistry
Agriculture
Resource type:
(Dataset) MS Peak table
Data source:
(Organism) Humulus lupulus
(Instrument) LC/MS-qToF
Software used:
Resource Viewing
Software:
R - unknown version
Alternative Software:
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Data processing:
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Publication year:
Rights holders:
Simon H. Steiner, Hopfen, GmbH
Funding:
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Uploaded by:
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Archiving date:
2020-10-13
Archive size:
189.6 MB
Archive creator:
1670fa88949cc897f41b44d58d08c11a
Archive checksum:
b11aed19432da5808a01001e9a0d9c57 (MD5)
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