Alternate identifier:
Related identifier:
Feiner, Alexander [Hopsteiner, Simon H. Steiner, Hopfen, GmbH, 84048 Mainburg, Germany & Leibniz Institute of Plant Biochemistry, 06120 Halle/Saale, Germany]
Pitra, Nicholi [Hopsteiner, S.S. Steiner, Inc., New York, New York 10065, USA]
Matthews, Paul [Hopsteiner, S.S. Steiner, Inc., New York, New York 10065, USA]
Pillen, Klaus [Martin-Luther University, 06120 Halle/Saale, Germany]
Wessjohann, Ludger A. [Leibniz Institute of Plant Biochemistry, 06120 Halle/Saale, Germany]
Riewe, David [Leibniz Institute of Plant Genetics and Crop Plant Research, 06466 Gatersleben, Germany & Julius Kuehn-Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Ecological Chemistry, Plant Analysis and Stored Product Protection, 14195 Berlin, Germany]
Additional titles:
(Abstract) Downy mildew in hop (Humulus lupulus L.) is caused by Pseudoperonospora humuli and generates significant losses in quality and yield. To identify the biochemical processes that confer natural downy mildew resistance (DMR), a metabolome- and genome-wide association study was performed. Inoculation of a high density genotyped F1 hop population (n=192) with the obligate biotrophic oomycete P. humuli led to variation in both the levels of thousands of specialized metabolites and DMR. We observed that metabolites of almost all major phytochemical classes were induced 48 hours after inoculation. But only a small number of metabolites were found to be correlated with DMR and these were enriched with phenylpropanoids. These metabolites were also correlated with DMR when measured from the non-infected control set. A genome-wide association study revealed co-localization of the major DMR loci and the phenylpropanoid pathway markers indicating that the major contribution to resistance is mediated by these metabolites in a heritable manner. The application of three putative prophylactic phenylpropanoids led to a reduced degree of leaf infection in susceptible genotypes, confirming their protective activity either directly or as precursors of active compounds.
downy mildew resistance
untargeted metabolomics
genome-wide association study
Related information:
Simon H. Steiner, Hopfen, GmbH, Mainburg, Germany
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany
Julius Kuehn-Institute (JKI), Berlin, Germany
Leibniz Institute of Plant Biochemistry (IPB), Halle (Saale), Germany
Production year:
Subject areas:
Resource type:
(Dataset) MS Peak table
Data source:
(Organism) Humulus lupulus
(Instrument) LC/MS-qToF
Software used:
Resource Viewing
R - unknown version
Alternative Software:
Data processing:
Publication year:
Rights holders:
Simon H. Steiner, Hopfen, GmbH
Name Storage Metadata Upload

Number of views in the previous six months.

Dataset page views




Overall statistics

Period Landing page accessed Dataset downloaded
Dec 2021 6 2
Nov 2021 13 0
Oct 2021 12 0
Sep 2021 23 0
Aug 2021 30 0
Jul 2021 23 4
Before 180 3
Total 287 9
Uploaded by:
Created on:
Archiving date:
Archive size:
189.6 MB
Archive creator:
Archive checksum:
b11aed19432da5808a01001e9a0d9c57 (MD5)
Embargo end date: